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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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STUDY OF THE ENUMERATION OF TWELVE CLINICAL IMPORTANT BACTERIAL POPULATIONS AT 0.5 MCFARLAND STANDARD
Authors
Gayathiri E,Bharathi B,Priya K
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Keywords
KEYWORDS: McFarland , Serial Dilution, Colony Count, Bacteria
Abstract
ABSTRACT:
McFarland standard is a specific scale for the concentration of bacteria /ml. It is used to approximate the number of microbial cells in the liquid suspension. The aim of the present study is to evaluate some clinically important bacterial inoculums with that compared to 0.5 McFarland standard and the bacterial inoculums were serially diluted by 10 fold dilution and spread plated and enumerating the number of colonies by help of colony counter and size of the organisms also been measured by micrometry. The results were read. As per McFarland rule there should be 15 colonies in the 7th dilution but all the species vary 15 times to three hundred times more than the expected value because of the size and volume of the organisms the time for the division also yield across amount in the suspension so the colony dilution may differ in each setup. The present study enumerated for 12 bacterial study has brought into light that the number bacterium in given sample could not be calculated by comparing it with McFarland standard because of varying number of population from 20- 324 any for the maintained bacteria
IJCRT's Publication Details
Unique Identification Number - IJCRT1807341
Paper ID - 190758
Author type - Indian Author
Page Number(s) - 880-893
Pubished in - Volume 6 | Issue 2 | July 2018
DOI (Digital Object Identifier) -
No Of Downloads - 1251
Author Country - 600042, india, chennai, chennai, india, Biological Science
Publisher Name - IJPUBLICATION | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
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Gayathiri E,Bharathi B,Priya K, "STUDY OF THE ENUMERATION OF TWELVE CLINICAL IMPORTANT BACTERIAL POPULATIONS AT 0.5 MCFARLAND STANDARD", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.6, Issue 2, pp.880-893, July 2018, Available at :http://www.ijcrt.org/papers/IJCRT1807341.pdf
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