Published Paper Details:

The unique physicochemical properties and interactions within the rhizosphere, copper-based nanomaterials (Cu-NMs) have been identified as efficient agents for boosting plant growth and improving nutrient absorption. This study investigates how Cu-NMs affect microbial activity in the plant rhizosphere, with a focus on how they encourage the synthesis of siderophores by helpful soil microbes. Compounds known as siderophores have a strong affinity for iron and help to solubilize and absorb this essential nutrient, which is essential for plant growth. According to our findings, when Cu-NMs are sprayed at the right quantities, they promote the development of microorganisms that produce siderophores, which raises the amount of iron available in the rhizosphere. Improvements in biomass accumulation, root growth, and chlorophyll levels were linked to the increased iron intake. This study presents a sustainable, nanotechnology-driven approach to increasing crop yield through microbiome-mediated nutrient uptake and provides fresh insights into the indirect channels through which Cu-NMs can improve plant nutrition. Flacourtia montana leaf extract was used in an environmentally benign reaction to produce copper nanoparticles. The ability of bacterial colonies to produce siderophores is demonstrated by the creation of yellow zones. By using a visual observation method, we can identify bacteria that improve the absorption of iron by plants. Evaluation of Cu- NMs-induced siderophore synthesis was made possible by the bacterial growth patterns and medium color changes seen during the study. The Debye-Scherrer equation analysis's 42.28 nm crystallite size result was consistent with the SEM results. The (111), (200), and (220) peaks in the X-ray diffraction investigation showed that the Cu2O nanoparticles have crystalline characteristics. Our scientific studies verified that significant increases in iron availability were brought about by copper nanoparticles and their microbial siderophore enhancing capabilities in rhizosphere circumstances.