Published Paper Details:

The present study was carried out to synthesize silver nanoparticles (AgNPs) using the aqueous extract of Tamarindus indica fruit shell through an eco-friendly green synthesis approach. The fruit shell extract was subjected to preliminary phytochemical screening and GC-MS analysis to identify the bioactive constituents responsible for nanoparticle synthesis. Silver nanoparticles were synthesized using silver nitrate solution and characterized by various analytical techniques including UV-Visible Spectroscopy, FTIR, XRD, SEM, EDAX, TEM, SAED, and DLS analysis. The formation of silver nanoparticles was confirmed by the characteristic color change from pale yellow to brown and by the appearance of a surface plasmon resonance peak in the UV-visible spectrum. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, tannins, phenols, saponins, terpenoids, glycosides, quinones, and carbohydrates. GC-MS analysis revealed several bioactive compounds with medicinal significance. Characterization studies demonstrated that the synthesized nanoparticles were predominantly spherical, crystalline, and well dispersed with nanoscale dimensions. The synthesized silver nanoparticles exhibited significant antioxidant activity as determined by DPPH radical scavenging assay, hydrogen peroxide scavenging assay, and total antioxidant capacity assay. Furthermore, the nanoparticles showed excellent antibacterial activity against both Gram-positive and Gram-negative bacterial strains, including Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae. The study confirms that Tamarindus indica fruit shell, an agricultural waste material, can serve as an effective reducing and stabilizing agent for the green synthesis of silver nanoparticles with promising biomedical and environmental applications.