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Professional grooming standards constitute a fundamental dimension of nursing professionalism, significantly influencing patient safety, therapeutic relationships, organizational image, and the overall quality of healthcare delivery. Adherence to appropriate grooming practices, including personal hygiene, professional attire, and institutional dress code policies, promotes infection prevention, reinforces professional identity, and enhances patients' confidence in nursing care. Nevertheless, disparities in nurses' knowledge, attitude, and practice regarding professional grooming standards continue to exist, potentially affecting the quality and consistency of clinical practice. The present study was undertaken to assess the knowledge, attitude, and practice regarding professional grooming standards among nurses working at a selected hospital in Chittoor District, Andhra Pradesh. A descriptive research design was employed, and 80 nurses were selected using a non-probability convenience sampling technique. Data were collected through a self-structured questionnaire and analysed using descriptive and inferential statistics. The findings revealed that a considerable proportion of nurses demonstrated inadequate knowledge, negative attitudes, and suboptimal grooming practices. Furthermore, knowledge scores showed statistically significant associations with selected demographic variables. The study underscores the necessity for structured educational interventions, effective institutional policies, and continuous professional development programmes to strengthen adherence to professional grooming standards and promote excellence in nursing practice.