Published Paper Details:

Enrofloxacin, a widely used fluoroquinolone antibiotic in veterinary medicine, requires stringent quality control to ensure its safety, efficacy, and stability. The development of stability-indicating analytical methods is essential for accurately quantifying the drug in the presence of its degradation products formed under various stress conditions. High-performance liquid chromatography (HPLC), particularly reverse-phase HPLC, has emerged as a reliable and sensitive technique for this purpose. This review focuses on the development and validation of stability-indicating HPLC methods for the quantitative estimation of enrofloxacin in pharmaceutical dosage forms. Key aspects of method development, including selection of chromatographic conditions, optimization of mobile phase, and detection parameters, are discussed in detail. The role of forced degradation studies under acidic, alkaline, oxidative, thermal, and photolytic conditions in establishing method specificity is also highlighted. Furthermore, the review summarizes validation parameters such as specificity, linearity, accuracy, precision, robustness, and sensitivity in accordance with International Council for Harmonisation (ICH) guidelines. Recent advancements in analytical techniques, including green chromatography and hyphenated methods, are also considered. Overall, stability-indicating HPLC methods play a crucial role in pharmaceutical quality assurance, regulatory compliance, and stability assessment of enrofloxacin formulations, ensuring the delivery of safe and effective veterinary products.