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Introduction A descriptive study to assess the assertive behaviour and self esteem of staff nurses in selected hospital in the city and to develop and disseminate an informational booklet regarding assertive behaviour. Objectives: To assess the assertive behaviour of staff nurses; to assess the self esteem of staff nurses; to associate the assertive behaviour of staff nurses with selected demographic variables; to associate the self esteem of staff nurses with selected demographic variables; to correlate assertive behaviour and self esteem and to develop and disseminate an informational booklet regarding assertive behaviour. Method: A non-experimental descriptive design was used to assess knowledge of 100 staff nurses. The participants were selected by using a non probability convenient sampling technique. The structured questionnaire was prepared to assess the knowledge of staff nurses. Results: It was found that most of the staff nurses 45(45%) were having assertive behaviour, 29(29%) of them were probably aggressive, 20(20%) were somewhat assertive behavior, 6(6%) were situationally non assertive, and none of the samples were very non assertive behavior. All of the staff nurses 100(100%) were having self esteem score "within normal range". It was also found that there was no significant association of assertive behavior with selected demographic variables, also there was no significant association of self esteem with selected demographic variables. Conclusion: The study concluded that most of the staff nurses 45(45%) were having assertive behaviour, 29(29%) of them were probably aggressive, 20(20%) were somewhat assertive behavior, 6(6%) were situationally non assertive, and none of the samples were very non assertive behavior. All of the staff nurses 100(100%) were having self esteem score "within normal range".