Published Paper Details:

Diazepam (Dz) is considered to be very safe initially, but later on it has been revealed that it may cause dependency. Since Dz is a drug of abuse, too, it may also cause intentional dependency. This intentional dependency is more serious due to the undefined nature of Dz consumption (Dose and frequency) and subsequent possible toxicity, which are generally not associated with therapeutic use of Dz. To evaluate the cellular toxic impact of Dz abuse, this study aimed to assess the impact of the number of defined overdoses of Dz on the cellular level of established enzyme markers for toxicity, i.e. TRAP-5b, ALT, AST, LDH and GST in the Swiss albino mice model. This study is unique as it monitors the serum level of these enzymes at various time intervals after discontinuation of Dz overdosing. The results of this study established a link between the number of Dz overdoses and irregularities in the serum levels of enzymes. It has been observed that the appearance of irregularities in the serum level of the chosen enzymes has been delayed after discontinuation of Dz abuse. Additionally, it is also evident that the persistence of this abnormality in the serum level of enzymes may depend on the number of doses of Dz (the higher the number of Dz overdoses, the longer the persistence of abnormalities). These irregularities are directly linked to Dz abuse with cellular toxicity as well as bone health.