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Aim: The study aims to find the Effect of Self-Instructional Manual (SIM) regarding clinical guidelines on phototherapy among staff nurses working in paediatric unit of selected Hospitals, Problem statement: Effect of Self Instructional Manual (SIM) regarding clinical guidelines on phototherapy among staff nurses working in paediatric unit of selected Hospitals, Maharashtra. Primary objective: To find out the effect of SIM regarding clinical guidelines on phototherapy among staff nurses working in paediatric unit of selected Hospitals, Maharashtra. Secondary objective: 1. To assess the knowledge of staff nurses regarding clinical guidelines regarding phototherapy before intervention. 2. To find out the effect of SIM regarding clinical guidelines on phototherapy after intervention. 3. To find out the association between posts test knowledge score regarding clinical guidelines on phototherapy and selected demographic variables of staff nurses. Method: Pre experimental with one group pre-test post-test design use for the study. It was conducted over 110 parents by using convenient sampling technique. Result: the pre intervention demographic variables of staff nurses were more or less similar revealing common characteristics. Further, it was observed that the percentages of knowledge (47.30%) on clinical guidelines on phototherapy among staff nurses were more or less similar before intervention. However, after an intervention, the percentage of knowledge on clinical guidelines on phototherapy was significantly increased from 47.30 % to 52.70 %. There was a significant difference (p<0.0001) between pretest and post-test knowledge scores on clinical guidelines on phototherapy. However, significant association (p>0.05) was found between knowledge on selected organ donation and age, gender, years of experience, qualification (higher education), designation. Findings of the study revealed that the self-instructional manual on clinical guidelines on phototherapy as a method was effective among staff nurses working in paediatric unit selected hospitals, Maharashtra. Interpretation and conclusion: From the findings of present study, it was concluded that the pre- intervention demographic variables of staff nurses were more or less similar revealing had similar characteristics. Percentage of knowledge and the mean scores of staff nurses were more or less similar before intervention. However, after an intervention, the percentage of knowledge and the mean scores of staff nurses with clinical guidelines on phototherapy were significantly increased. There was a significant difference between pretest and post-test knowledge scores. Thus, it was concluded that the SIM on clinical guidelines on phototherapy as a method of teaching was effective among staff nurse working in pediatric unit selected hospitals, Maharashtra.