Published Paper Details:

Abstract Cassia fistula L., commonly known as the golden shower tree, is a well-documented medicinal plant in traditional Ayurvedic medicine. This study aimed to extract and characterize the phytochemical constituents of Cassia fistula seed oil and evaluate its biological activities, including antioxidant and antimicrobial properties. The seed oil was extracted using Soxhlet extraction with n-hexane and analyzed through preliminary phytochemical screening, GC-MS (Gas Chromatography-Mass Spectrometry), and FTIR (Fourier Transform Infrared Spectroscopy). The GC-MS analysis revealed the presence of bioactive fatty acids such as oleic acid, linoleic acid, and palmitic acid, among other phytoconstituents with potential pharmacological properties. FTIR spectra confirmed the presence of functional groups like hydroxyl, carbonyl, and aliphatic chains. Biological evaluation of the oil demonstrated significant antioxidant activity in DPPH, ABTS, and FRAP assays, with IC50 values comparable to standard ascorbic acid. The antimicrobial activity was tested against both Gram-positive and Gram-negative bacteria, with moderate to strong zones of inhibition observed against Staphylococcus aureus and Escherichia coli. The findings suggest that Cassia fistula seed oil is a promising natural source of bioactive compounds with potential applications in pharmaceutical, nutraceutical, and cosmetic formulations.