Published Paper Details:

In India, Guilandina bonduc is a common plant. Because this plant is frequently utilised in traditional medicine, the biotechnology of endophytic fungi is extremely important because natural resources have decreased of plants. The endophytic fungus that were discovered in Guilandina bonduc plant and produced a variety of chemicals were described in this study. The endophytic fungal species were identified by morphological and molecular means using phylogenetic tree analysis. The cultivation method was carried out using Potato Dextrose Broth media. The extraction and evaporation processes were carried out using a rotary evaporator by using ethyl acetate as a solvent. The DPPH method and paper disc diffusion method were used to conduct antioxidant and antibacterial testing. The compound was isolated using chromatographic methods, and its chemical structure was determined by spectroscopic examination. Lasiodiplodia irregularis was determined to be an endophytic fungus based on the morphological and molecular study results. 2-Nonynoic acid was the pure chemical that was extracted from this endophytic fungus. This substance was probably going to be used as a starting point for novel antioxidants.