Published Paper Details:

ABSTRACT: This study aims to develop an analytical methodology for the concurrent estimation of Fipronil and Methoprene. This involved the creation and validation of both RP-HPLC and UV spectrophotometric methods suitable for analyzing these drugs in their pharmaceutical formulations. The UV spectrophotometric method employed a Q-absorption ratio approach. This particular method relies on absorbance measurements at two specific wavelengths: the iso-absorptive point (identified as 231 nm for Fipronil and Methoprene in methanol) and the ?max of Methoprene, one of the two components. The maximum wavelength was at 264nm which was of methoprene. Fipronil and Methoprene both showed linearity in the concentration range of 1-10 mg/ml. Using a C11 BDS column (100 mm 4.6 mm), the HPLC separation was accomplished. Employing a mobile phase made of acetonitrile and water in an 80:20 volume ratio. At a maximum wavelength of 254 nm, the analyte was detected while the mobile phase was flowing at a rate of 1.5 ml/min. In samples, the drug was eluted from the column after 3.1 minutes, and after 4.71 minutes. To estimate recovery and linearity, five different concentration levels of 5, 10, 15, 20, and 25 g/ml were used. The % RSD findings were found to be <2% for both the methods and the correlation coefficient was more than 0.999.