Published Paper Details:

Abstract: Parmotrema perlatum (commonly known as black stone flower/kalpasi) is a foliose lichen traditionally used in India and recognized as a source of bioactive secondary metabolites. In this study, GC-MS profiling of P. perlatum extracts identified Thujopsene and Resibufogenin as major constituents. Antibacterial screening against Bacillus species revealed only weak inhibition, suggesting that active metabolites occur at low concentrations in crude extracts. To complement these results, molecular docking was performed using AutoDock Vina against representative bacterial proteins, including D, D-peptidase Rv3330 (Mycobacterium tuberculosis), Cag? ATPase (Helicobacter pylori), dihydrofolate reductase (Staphylococcus aureus), and DL-endopeptidase CwlO (Bacillus subtilis). Among the tested compounds, Resibufogenin displayed consistently high binding affinities across multiple targets, underscoring its promise as a potential antibacterial lead molecule. Overall, this work demonstrates the value of integrating in vitro assays with computational modeling and highlights P. perlatum as an underexplored source of novel antimicrobial agents requiring further isolation, characterization, and preclinical validation.