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ABSTRACT AI chatbots are being used more and more in the fields of education and healthcare since they enable self-directed learning and offer 24/7 access to health information. The purpose of this study was to evaluate how nursing students viewed AI chatbots as a tool for health education. OBJECTIVES OF THE STUDY 1. To assess the perception of nursing students regarding the use of AI chatbots for health education. 2. To determine the association between perception levels and selected demographic variables. Methodology A descriptive cross-sectional study was conducted among 100 B.Sc. Nursing students (1st and 4th year) at St. Peter's Nursing College and Research Institute, Hosur. A structured questionnaire comprising demographic data and a 20-item Likert scale was used to assess perceptions. Convenience sampling was adopted. Data were analyzed using descriptive and inferential statistics, including Chi-square tests. Results: Out of 100 students, 64% had a positive perception, 35% had a neutral perception, and 1% had a negative perception of AI chatbots in health education. A significant association was found between access to technology (p < 0.05) and the use of AI chatbots for educational purposes (p < 0.05), as well as perception levels. No significant association was found with other demographic variables. Conclusion: The findings suggest that AI chatbots are generally well accepted as effective educational tools among nursing students. With appropriate integration into the curriculum and training on digital literacy, AI chatbots can enhance nursing education.