Published Paper Details:

Abstract: : A simple, precise, and accurate stability- indicating rear phase high- performance liquid chromatographic( RP- HPLC) system was developed and validated to graphy for the contemporaneous estimation of Captopril and Hydrochlorothiazide in bulk and combined lozenge form. The chromatographic separation was achieved using a C18 column with a suitable mobile phase composed of( insert exact composition, e.g., acetonitrilebuffer( pH 3.0) in a defined rate), at a inflow rate of( insert inflow rate) mL/ min and discovery wavelength at( insert discovery wavelength, e.g., 220 nm). The system was validated as per ICH guidelines for parameters including linearity, delicacy, perfection, particularity, limit of discovery( LOD), and limit of quantification( LOQ). Forced declination studies were conducted under colorful stress conditions like acid, base, oxidation, thermal, and photolytic to establish the stability- indicating nature of the system. Both medicines were well resolved with satisfactory retention times, and no hindrance from declination products was observed. The developed RP- HPLC system proved to be dependable, robust, and suitable for routine quality control analysis of Captopril and Hydrochlorothiazide in both bulk medicine and pharmaceutical phrasings.