Published Paper Details:

As sequencing of DNA technologies have evolved across multiple generations, the science of genomics has undergone a major revolution. Starting from earliest first-generation Sanger method sequencing to the emergence of second- and third-generation approaches, this account traces the course that culminated in the innovative development of nanopore sequencing. With each generation, the speed, read length, accuracy, and cost-effectiveness improved. A salient characteristic of nanopore sequencing is its real-time, direct reading of long DNA or RNA strands without needing to tag or amplify them. The portability, simplicity, and flexibility of the technology have made possible applications across a range of fields, such as clinical diagnostics, infectious disease surveillance, agriculture, forensic studies, and personalized medicine. The underlying operation of solid-state and biological nanopores is also explored within this book, with a focus placed on their utilization within modern sequencing platforms. The review also includes advances that aid us in understanding genomic complexity better, such as RNA-seq, single-cell analysis, and long-read and short-read sequencing. Genomics is becoming increasingly accessible, effective, and impactful with the application of nanopore technology, opening the way for revolutionary discoveries in the areas of health, the environment, and more.