Published Paper Details:

The clinically important Salmonella include Salmonella typhi, Salmonella paratyphi- A, and Salmonella paratyphi-B; which cause typhoid fever, paratyphoid A, and B fever, respectively. For this, the leaves of Enicostemma axillare were selected. The antityphoidal activity of extracts were studied using standard procedures. The antityphoid activity test performed using the agar disk diffusion method revealed that all four solvent extracts showed activity against S. typhi and S. paratyphi-A. The highest zones of inhibition were obtained from the ethanol extract of leaves with diameters of 16.00+0.57 mm and 14.16+0.44 mm at 100 mg/ml, for S. typhi and S. paratyphi-A respectively. The leaf extracts of Enicostemma axillare showed significant antityphoid activity which can be used as a remedy against the Salmonella typhi and Salmonella paratyphi-A and therefore promise to be effective antityphoidal supplement for typhoid patients. The antityphoidal activity of the leaf extracts were compared with azithromycin 17.66+0.88 mm and 19.00+0.57 mm zone of inhibition for S. typhi and S. paratyphi-A respectively. The present results indicate that the leaf extracts of Enicostemma axillare has antityphoid potential against both typhoid pathogenic strains i.e. Salmonella typhi and Salmonella paratyphi-A. The Minimum inhibitory concentration (MIC) of water, ethanol, chloroform and hexane extracts of leaf extracts were determined by broth dilution method. The water, ethanol and chloroform extracts of Enicostemma axillare leaf showed 12.5mg/ml MIC against both strains Salmonella typhi and Salmonella paratyphi-A.