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Abstract: Vitamin D deficiency is a widespread and serious global health concern, affecting a remarkable 30-80% of children and adults worldwide, regardless of a country's developmental status. The well-documented role of vitamin D in bone mineralization and skeletal health is complemented by a growing body of evidence linking vitamin D deficiency to a range of chronic diseases, including type 1 diabetes mellitus, systemic lupus erythematosus, multiple sclerosis, cardiovascular disease, and various malignancies.3 Notably, research has consistently revealed disturbingly high prevalence rates of vitamin D deficiency and insufficiency among adolescents globally, even in countries with abundant sunshine, which is the primary catalyst for endogenous vitamin D synthesis.4 This widespread deficiency among adolescents, typically considered a healthy demographic, underscores the need for increased awareness, education, and preventive measures to minimize the long-term consequences of vitamin D deficiency. Adolescence is a critical period of growth and development, and vitamin D plays a crucial role in maintaining strong bones, immune function, and overall health. Adequate knowledge about vitamin D deficiency is essential for adolescents to make informed decisions about their health. However, existing research suggests that many adolescents lack a clear understanding of vitamin D deficiency, its risk factors, symptoms, and prevention strategies, highlighting a significant knowledge gap that needs to be addressed. Aim : To evaluate the level of awareness and understanding about vitamin D deficiency among the adolescent students and identify knowledge gap. Objectives : 1. To assess the knowledge regarding vitamin D deficiency among adolescent students 2. To determine the association between the knowledge of vitamin D deficiency with selected demographic variables Methods : Descriptive study was conducted in Kajiranga English Academy, Guwahati, Assam. 100 samples were drawn by using non probability convenient sampling technique. The knowledge of the students were assessed by using a structured self-administered questionnaire . Results : The study result reveled that out of 100 students , majority of the students ie. 33 (33%) falls under the age group 16-17 years, 61(61%) students are male, 29 students (29%) are from class 11 , 37 students (37%) spends 1-2 hours in outdoor, 80 students (80%) are non-vegetarian , 68 students (68%) previous knoeledge regarding vitamin D deficiency and out of that majority 32 students (47.06%) have knowledge from internet sources . The study also showed that out of 100 students, majority of the students 70 (70%) have moderate knowledge, 19(19%) have inadequate knowledge and 11(11%) have adequate knowledge on vitamin D deficiency . The study findings states that there is significant association between level of knowledge on vitamin D deficiency and demographic variable (presence of previous knowledge) at p= <0.05 level and with the mean score of knowledge 8.82+3.09. The calculated Karl Pearson's correlation "r" value was 0.87 . It also reveals that there is no significant association with other demographic variables such as Age, Gender, Educational qualification, food preference , time spend outdoor, presence of previous knowledge on vitamin D deficiency Conclusion: Based on study findings, it is concluded that there is a need to improve the concept regarding vitamin D deficiency among the adolescent students . The study underscores the significance of vitamin D deficiency as a public health concern and highlights the need for multifaceted interventions to address its prevalence and consequences.