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Abstract This study aims to compare the effect of unilateral and bilateral plyometric training in combination with resisted sprint on sprint kinematics adolescent boy' sprinters speed, flight time and ground contact time. Forty-five moderately trained subjects, aged 13 to 15 years studying in different schools from Idukki and Kottayam district, of Kerala state, India were selected as subjects. The selected subjects divided into three groups, namely, Unilateral Plyometric training in Combination with Resisted Sprinting (UPTCRS) Group I (n = 15), Bilateral Plyometric Training in Combination with Resisted Sprinting (BPTCRS) Group II (n = 15) and (CG) Control Group III (n=15). Both the groups performed maximal effort of plyometric leg exercises and resisted sprinting for 3 days a week for 12 weeks. Group I performed all the exercises with full repetition of each limb subsequently change to other side of the limb, left limb followed by right limb. Group II performed all the exercises with both limbs simultaneously. The data were analysed by using SPSS version 20, paired 't' test for each group, and ANCOVA were used to find out the effect between the groups. The paired mean differences were determined using Scheff's post hoc test whenever the adjusted post-test 'F' ratio values were found to be significant. Results of the study shows that unilateral and bilateral plyometric training in combination with resisted sprinting were significantly improved speed, flight time and ground contact time. Results of the study shows unilateral and bilateral plyometric training in combination with resisted sprinting were significantly improved on speed. Speed 't' ratio of UPTCRS, BPTCRS and CG were 3.09,3.59 and 0.140 (4.49%, 4.52% and 0.01%) . Flight time 't' ratio of UPTCRS,UPTCRS and CG were 9.13,7.93 and 1.87 and (11.81%,8.67% and 0.16%. Ground contact timeT 't' ratio of UPTCRS,BPTCRS and CG were 12.13,10.66 and 1.46 (18.04%,14.39% and 1.51%). ( P< 0.01).The conclusion of the study stated that the speed ,flight time and ground contact time of the unilateral and bilateral plyometric training in combination with resisted sprint training group subjects were significantly increased when compared with the control group. However, when comparing the experimental groups, bilateral plyometric training in combination with resisted sprinting group was better than unilateral plyometric training in combination with resisted sprinting group.