Published Paper Details:

Method development and validation are critical components of analytical chemistry, particularly for UV-Visible (UV-Vis) spectroscopy and High-Performance Liquid Chromatography (HPLC). These techniques are widely used for the qualitative and quantitative analysis of pharmaceuticals, environmental samples, and food products. Over the past decade, advancements have focused on improving accuracy, precision, robustness, and sensitivity in analytical methods. For UV-Vis spectroscopy, the emphasis has been on optimizing wavelength selection, solvent compatibility, and linearity in absorbance-concentration relationships. Enhanced chemometric approaches and advanced software tools have streamlined method development, reducing time and improving reliability. In HPLC, method development has centered around optimizing chromatographic conditions such as mobile phase composition, flow rate, column selection, and detection methods. Novel stationary phases, gradient elution techniques, and the integration of mass spectrometry have expanded HPLC's applicability. Validation strategies, guided by ICH (International Council for Harmonisation) guidelines, ensure the reliability of methods through rigorous testing of parameters like accuracy, precision, linearity, limit of detection, and limit of quantification