Published Paper Details:

Verapamil is used to treat high blood pressure, and there is interest in creating a new HPLC method to measure the drug concentration and distribution in rat bodies. Using a gradient elution technique with a buffer containing 1 mL of FA in 1 L of HPLC-grade water and a mixture of two components, such as buffer and ACN, in a 60:40 ratio as the mobile phase at a flow rate of 1 mL/min over a good linear concentration range, chromatographic separation was accomplished on a Symmetry C18 column. The outcomes of the recovery study, precision, matrix effect, and extraction recoveries are all within an acceptable range. This technique was used to distribute verapamil throughout the rat's head, body, and tail. In accordance with UFDA rules, we discovered that the medications remained stable throughout the stability tests. Verapamil bioavailability experiments have been conducted effectively for the first time using the proven technique