Published Paper Details:

Senna occidentalis L. is a medicinal plant known for its traditional use in various therapeutic applications. In this study, we aimed to elucidate the metabolite profile of Senna occidentalis L. root extract using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). The phytochemical composition of the extract was analyzed to identify and characterize the bioactive compounds present. The GC-MS analysis revealed a diverse array of volatile and non-volatile compounds in the Senna occidentalis L. root extract, including alkaloids, terpenoids, flavonoids, and other secondary metabolites. LC-MS/MS further provided a comprehensive identification of the polar and non- polar constituents, allowing for a more in-depth understanding of the chemical composition. To explore the potential therapeutic applications of the identified compounds, the antimicrobial activities of the Senna occidentalis L. root extract was evaluated against a panel of pathogenic microorganisms. The extract exhibited notable antimicrobial effects, demonstrating its efficacy against both Gram-positive and Gram-negative bacteria, as well as fungi. The findings from this study contribute valuable insights into the chemical composition of Senna occidentalis L. Roots and highlight its potential as a source of bioactive compounds with antimicrobial properties. Further research is warranted to isolate and characterize specific compounds responsible for the observed activities, paving the way for the development of novel pharmaceuticals or natural products for therapeutic applications.