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The progress in medical science has been significantly propelled by the advancements in nanotechnology, offering ground breaking solutions to combat life-threatening diseases. Nanotechnology represents a significant milestone with diverse applications across various sectors such as electronics, textiles, and particularly in healthcare. Nanotechnology is pivotal in healthcare due to its significant contributions in precision medicine applications such as targeted therapy, diagnostic techniques, therapeutic interventions and biomedical sensing all of which enhance human well-being. Nanoparticles serve as an extremely promising framework for a wide range of biomedical uses, showcasing their immense potential in revolutionizing medical treatments and interventions. The emergence of eco-friendly technologies for the biosynthesis of nanoparticles represents a significant stride within the realm of nanotechnology. This development intersects with the broader domain of materials science, particularly in the manipulation of atomic structures to confer distinct properties and facilitate diverse bio-applications. Among metal nanoparticles, silver nanoparticles stand out due to their remarkable physical, chemical and biological attributes. In this context, green chemistry has surfaced as a viable alternative to conventional synthesis methods for nanoparticles. Among the array of green approaches, leveraging plant extracts for nanoparticle synthesis garners particular attention. This preference stems from the rich diversity of biomolecules inherent in plants, which serve not only to reduce nanoparticles but also to act as stabilizing and capping agents, thereby expediting reaction kinetics. In contrast to microbial cultures, plants offer ease of handling, wide distribution and ready availability. The present review delves into the varied plant species suitable for rapid, one-step protocols in silver nanoparticle synthesis. Additionally, it delineates the multifaceted bioactivities exhibited by these nanoparticles, encompassing antibacterial, antifungal, antioxidant, antiviral, anticancer and anti-diabetic properties.