Published Paper Details:

Mastitis is the most common disease in dairy cows and buffaloes and has well-documented negative effects on animal welfare and dairy farm profitability. Antimicrobial treatment of infection can help to eliminate or reduce the occurrence of mastitis infection. The widespread use of antibiotics raised concerns about the emergence of antibiotic-resistant pathogens, prompting the dairy industry to reduce antibiotic use. As a result, alternative therapies for the prevention and treatment of bovine mastitis, particularly natural products derived from plants and animals, have been sought. Escherichia coli (E. coli) is a highly adaptable bacteria that may develop biofilms in certain situations. This study aims to explore the virulence character like biofilm production and resistance to antimicrobials of E. coli which are isolated from the bubaline mastitis milk samples. 16 milk samples from buffaloes with mastitis in coastal Andhra Pradesh were used in the current study. The original biochemical assays used to identify these isolates were the Indole test, Methyl red, Catalase, Spot Oxidase, Voges-Proskauer, Haemolytic activity on 5% Sheep Blood agar, and Biofilm Activity on Congo Red Agar. By using the Kirby-Bauer disc diffusion technique, the susceptibility of six antimicrobials to verified E.coli isolates was evaluated. By using biochemical techniques, 5 samples from the 16 isolates were tentatively positive for E. Coli. The drug with the greatest level of resistance was penicillin (100 %). The findings indicate that the organisms are able to produce biofilm. They are rapidly gaining genes that make them resistant to the standard antibiotics used to treat mastitis. In vitro study demonstrates the efficacy of penicillin against gram-positive mastitis pathogen is high.