Published Paper Details:

Blotting is a classical technique for detecting macro-biomolecules of interest from a mixture of molecules. The technique is generally composed of four steps: 1) gel electrophoresis-based separation of molecules, 2) blotting (transfer and immobilization) of separated molecules from the gel onto the membrane, 3) specific hybridization of probes to target molecules on the membrane, and 4) visualization of the probe/target molecule complex. The present article briefly introduces blotting techniques for readers, since the direction-named techniques are confusing for people beyond the biotechnology field. Particularly, the smartness of the blotting step in a blotting technique is emphasized. Specifically, the blotting step allows for fixation of size-separated molecules on the membrane, and permits subsequent hybridization of the target molecule to labeled probe, collectively allowing target molecules to be detected by imaging. In this article, we will discuss about the blotting techniques, their principles and fabulous procedures. Blotting techniques play a pivotal role in molecular biology, enabling scientists to analyze and visualize biomolecules with precision. These techniques have revolutionized the way we study DNA, RNA, and proteins, offering valuable insights into cellular processes. In this article, we will explore the fundamentals of blotting techniques, their applications, and advancements in the field. We will also provide references to learn and acknowledge the importance of blotting techniques.In conclusion, not only is blotting a smart strategy for enabling the detection of molecules of interest but also the concept of blotting plays an important role in biotechnology.