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Medicinal plants are assuming greater importance in the primary health care of individuals and communities in many developing countries. There has been an increase of demand in international trade because of very effective, cheaply available, supposedly have no side effects and used as alternative to allopathic medicines. Medicinal plants are believed to be much safer and proved elixir in the treatment of various ailments. Rhizome and roots of this plants are used as tonic in typhoid fever and dyspepsia. Traditional use of this drug is in diarrhea, typhoid, jaundice, fever, headache, skin disease and syphilis. In another combination of drug, Drynaria is used for expelling rheumatism. In the treatment of hyperthyroidism, Drynaria along with other drugs are used. The ethnomedicinal uses of the fern Drynaria quercifolia have been pharmacologically confirmed by several workers such as anthelmintic activity, antibacterial and antidermatophytic activity. Hence the objective of this present study is to estimate the anti-inflammatory activity & anti-diabetic activity of the fresh and Cabinet dried drynaria quercifolia (l.)j. sm. -polypodiaceae . In-vitro anti-inflammatory activity of the fresh and Cabinet dried were carried out by heat induced hemolysis method. In-vitro anti-diabetic activity of the fresh and Cabinet dried drynaria quercifolia (l.)j. sm. -polypodiaceae were carried out by ?-amylase method. Cabinet dried drynaria quercifolia (l.)j. sm. -polypodiaceae have shown a better profile of anti-inflammatory & anti-diabetic activity compared to fresh drynaria quercifolia (l.)j. sm. -polypodiaceae and thus it can be used for therapeutic purposes. Results revealed that the Drynaria quercifolia rhizome extracts contains abundant amount of anti-inflammatory and anti-diabetic activity.