Published Paper Details:

LC-MS is a technique that combines physical separation capabilities of liquid chromatography with mass analysis compatibilities of mass spectrometry. It is a method that combines separation power of HPLC with detection power of mass spectroscopy. In LC-MS we remove detector from the column of LC and fit the column to interface of MS In the most of the cases the interfaces used in LC-MS are ionization source. The basic theory of HPLC is for separating a complex mixture into its components. High sensitivity of mass spectroscopy provides the information for identification compounds. As the metabolites appears the end of the column, they enter mass detector where the solvent is removed and metabolites are ionized. The use of LC-MS in toxicology laboratory is for drug conformation testing of an immune assay screen and for broad spectrum drug screening. Multiple different LC-MS platform including LC-TOF and LC-orbitrap have been used for toxicology testing. In addition, multiple different data acquisition modes have been described including target, Acquisition methods with & without data dependent acquisition of product ions spectra. This chapter highlights two LC-MS applications including opioid conformation testing, broad-spectrum drug screening laboratories experience with method development, validation and implementation.