Published Paper Details:

Since the first DNA extraction by Friedrich Miescher in 1869, scientists have made extraordinary strides in developing extraction methods that are more reliable, easier and faster to perform, less expensive, and yield higher yields. A fast, simple, and inexpensive DNA extraction method with greater yield and purity of DNA would aid in rapid diagnosis, large population screening, and other routine PCR-based applications. The present study evaluated and standardized a rapid and zero-cost DNA extraction from targeted organism for routine molecular studies. Four DNA extraction methods i.e. 1.4 M sodium chloride (NaCl), 20 mM ethylene diaminetetraacetic acid (EDTA, pH 8.0), and elution from blotted nitrocellulose membrane (NCM) were compared with standard CTAB extraction buffer, EtBr-CsCl Gradient Centrifugation Method and salting-out method is a non-toxic DNA extraction method. There has been an increasing demand for reliable and efficient DNA isolation methods that can yield sufficient amounts of high quality DNA with minimal contamination. The current review addresses the development of different DNA extraction techniques from solvent-based methods to physical extraction methods, each with their distinct advantages and limitations.