Published Paper Details:

Phytosomes of neem leave extract has been successfully formulated in this procedure firstly Azadirachta indica leaves were morphological Characterized. Neem leave powder was subjected for various physiochemical analysis then extracted out methanolic fraction of neem leaves, yield of the extract which was 6.34 %. Phytochemical screening found that carbohydrate, alkaloids and flavonoids were present. then extracted out methanolic fraction of neem leaves, yield of the extract which was 6.34 %. Phytochemical screening found that carbohydrate, alkaloids and flavonoids were present. Total phenolic compound (TPC) was 0.756mg/100mg of gallic acid equivalent of dry extract sample, Total alkaloid content was atropine equivalent 0.632mg/100mg. Five different type of phytosome formulations were formed using different ratio of Phosphatidylcholine and Cholesterol (4:1), (2:1), (4:3), (1:1) and (1:1.25). These formulations were characterized under various parameters like yield, drug content, particle size and Encapsulation Efficiency. For all formulation yield was 88.32 % to 98.91%, drug content was 90.21% to 97.52 %, Mean Particle Size(nm) about 700 nm and Encapsulation Efficiency was 78.67% to 95.34%. Drug: Excipient Compatibility confirmed by FT-IR studies. All five phytosome formulations were incorporated with gel and evaluated under the various parameter, pH of all formulations was observed between 6.8 to 7.3 and Spreadability between 5.6 to 7.9 cm. % drug content between 98.9 % to 101%, and viscosity between 98 to 115 centi poice (cp) and % permeation between 83.2 % to 92.7 %. But on the basis of drug release kinetics F-3 formulation was very good because its % drug release was 97.913% followed Higuchi Kinetic Model. With the 95.56% drug release F-2 formulation was good both follow First order Kinetics.