Published Paper Details:

This research paper presents a study on the simultaneous quantification and force degradation analysis of Remogliflozin, Evogliptin, and Metformin using the hydrophilic interaction liquid chromatography (HILIC) technique. The solubility of the three drugs was determined, and acetonitrile was selected as the solvent for further analysis. The wavelength of 210 nm was chosen for analysis due to the highest absorption peak intensities. Stock standard solutions were prepared for each drug, and working standard solutions were then prepared for the quantification studies. The HILIC technique, specifically using the Acclaimed Mix-Mode HILIC-1 column, was employed for the separation of the analytes. Various mobile phases were tested, and the optimized chromatographic conditions were determined. The linearity of the method was evaluated, and calibration curves were constructed for each drug. The accuracy of the method was assessed by analyzing samples from marketed formulations, and the results showed high drug recovery within the acceptable range. The validation parameters, including accuracy, precision, sensitivity, robustness, specificity, and selectivity, were evaluated according to ICH guidelines. The developed HILIC method demonstrated reliable quantification of Remogliflozin, Evogliptin, and Metformin in bulk samples and exhibited suitable analytical performance for future applications.