Published Paper Details:

A method to find and examine the fluorescence in the sample is fluorescence spectroscopy, fluorimetry, or spectrofluorimetry. An energy shift from a higher to a lower state that is accompanied by radiation is known as fluorescence. When an electron transitions from an excited state to the electronic ground state and releasesits excess energy as a photon, the process is known as luminescence, which is the emission of light by a substance. Fluorescence spectroscopy is a rapid and accurate method for understanding the molecular environment and events in materials. Because of its remarkable sensitivity, high specificity, simplicityof use, and low cost fluorometry is favored over other analytical techniques. It is a reliable technique that is frequently applied in the field of medical diagnosis for a variety of environmental applications. This review portrays a concise account of the introduction to spectrofluorimetry, its principle, factors affecting and its variousapplications in organic, inorganic, agricultural, chemical, industrial, pharmaceutical, biosciences, environmental and specific applications are also includedin this article.