Published Paper Details:

Aquaculture is one of the fastest growing sector in the world, especially in Asia. it has become an important economic activity for many countries. Various kind of diseases are the major problem associated with this sector it reduces the yield considerably. Usually antibiotics are used against diseases. But antibiotic administration has many problems; to overcome these problems probiotic can be used as an alternative.Anatagonestic activity is a major characteristic of probiotics. Probiotics produce an array of antimicrobial compounds , so the location and characterization and effect of these compounds against the fish pathogens are very much beneficial. The compounds may be present either intracellularly or extrecellularly. So the antimicrobial activity determination of all these components is necessary. For that 4 cellular components such as intracellular product(ICP), extracellular product(ECP), heat killed whole cell product(HKWCP), and whole cell product(WCP) can be used, and their antimicrobial activity pattern determination and comparison of this with standard antibiotic will show their activity. Then the highest activity showing fraction then subjected to various analysis to identify which is the component which contributes this effect; for that Dialysis, Tricine SDS -PAGE, TLC analysis etc can be done . After that the effective component should be used for some field trails to find whether thy are effective in invivo conditions or not.