Published Paper Details:

Many organic solvents namely butanol, acetic acid, ethyl acetate, chloroform, cyclohexane, methanol, ether, acetonitrile, toluene, dichloromethane, benzene and carbon tetra chloride are used to perform thin layer chromatography (TLC) of various drugs. Most of these organic solvents are harmful to health and are expensive. Some of these organic solvents are carcinogenic. Organic solvents are responsible for polluting the environment also their disposal from laboratories, industries etc. require special procedure which makes the process more tedious and costly. In the present research work mixed hydrotropic solutions were used as mobile phases to perform TLC of the drugs, precluding the use of organic solvents. Ciprofloxacin hydrochloride, Thiamine hydrochloride, Metformin hydrochloride, Lignocaine hydrochloride were selected as model drugs. Sodium benzoate, sodium citrate, sodium acetate and urea in various combinations were used as model hydrotropic agents. The solutions of above mentioned hydrotropic agents in distilled water in different concentrations were used as mobile phases to perform TLC of selected drugs. The Rf values so obtained in the proposed methods ranged from 0.19 to 0.84. As per mixed solvency concept, all molecules present in this universe possess solubilizing properties. In this particular research, a green method has been explored utilizing the solubilizing properties of solids. We can replace harmful organic solvents in this way.