Published Paper Details:

The present study was designed with the main aim of detection of variations of somaclones in the banana cv. Grand Naine (AAA) using sequence related amplified polymorphism (SRAP) molecular marker technique. Matured leaves that are free from damage caused by pests or disease were collected in brown covers from different field places Karnataka state. Isolation of genomic DNA was carried out by cetyl trimethyl ammonium bromide. The integrity and purity of DNA and visualization of amplified products was done by electrophoresis and quantified. PCR amplification of DNA from banana somaclones using SRAP markers were carried out using Taq DNA polymerase and short stretch of oligonucleotides (primers), which are specific to the DNA to be amplified. Then the SRAP, markers are used to assess the genetic variation in somaclonal variants of banana cv. Grand Naine (AAA). The amplified products of SRAP markers were separated using agarose gel electrophoresis technique. out of 44 primers combination used, only 8 combinations showed polymorphism. The 8 primer combinations showed very good intense polymorphic bands. SRAP primer combinations EM3+ME3, EM2+ME6, EM5+ME5, EM7+ME7 showed clear polymorphism. In conclusion, overall 19.4% polymorphism between dwarf off type and the normal banana cv. Grand Naine (AAA) plants was obtained.