Published Paper Details:

Predominant microalgae, Chlorella vulgaris, Acutodesmus nygaardii and Scenedesmus armatus were isolated from three selected Thermal power stations namely, Vallur Thermal power station (VT), Tamil Nadu, Kolaghat Thermal power station (KoT), West Bengal and Khapergada Thermal power station (KhP), Maharashtra, India. These strains were cultured in BBM medium. Gene specific primer for carbonic anhydrase gene in the genomic DNA of the three selected microalgae (Chlorella vulgaris, Acutodesmus nygaardii and Scenedesmus armatus) were used and were also expressed in all the thee organisms studied and which further confirms the presence of Carbonic anhydrase gene and the ability of those organisms to produce this particular enzyme which catalyzes the reversible conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ?HCO3- + H+) with very high efficiency. Amount of Carbonic anhydrase enzyme produced in media with and without 0.1M NaHCO3 were analyzed. The enzyme was extracted from the harvested biomass of Chlorella vulgaris, Acutodesmus nygaardii and Scenedesmus armatus. The molecular weight of the purified carbonic anhydrase was reported as 30 kDa and there were a band formations occurred at that particular range for all the three chosen microalgae.