Published Paper Details:

An efficient in vitro callus induction protocol was developed for the first time in Gymnema hirsutum Wight & Arn . which is a potent antidiabetic plant.shoot tips and nodal explants were inoculated on MS medium fortified with auxins (2,4-D and NAA) and cytokinins (kinetin) different concentrations ranging from 1.0 to5.0 mg/l in alone or in combinations for rapid callus induction. Callus was obtained in both explants in all the concentrations. These explants failed to produce callus on MS medium without growth regulators. The highest callus induction , light/dark green with campact/friable nature and growth rate (fresh and dry weights) was observed on MS media containing 2,4-D(3.0mg/l) + NAA(1.0 mg/l) followed by 2,4-D and NAA at 3.0 mg/l. but kinetin is less effective for callus induction than the 2,4-D and NAA . The higher concentrations of 2,4-D (4.0 and 5.0 mg/l) callus was turned to brown colour and necrotic . It is concluded that, in addition to the plant material, callus may also used as a supplement raw material to obtain secondary metabolites for the pharmaceutical industry.