Published Paper Details:

: Protein can be quantified by various biochemical assays but all methods are subjected to artifacts. Also, the nature of the sample governs the selection of appropriate method. Fish visceral extract is a complex mixture of bioactive peptides, polypeptides, lipoproteins, hexopeptides etc. Samples containing higher protein concentration vary the results of Bradford assay. On the other hand, Lowry assay and Biuret assay suffer artifacts by the presence of non-proteinaceous components. In this study, protein content in liver and intestinal extracts of Catla catla and Labeo rohita was determined by the methods of Lowry et al. (1951), Gornall (1949) and Bradford (1976). Highest protein content was found in Catla liver by all the methods (1740 mg ml-1, 580 mg ml-1 and 1450 mg ml-1 resp.). Lowest concentration was found in Catla intestine by all the methods (1290 mg -1 ml, 200 mg ml-1 and 1030 mg ml-1, resp.). A good association (98.6%) was found among the three methods indicates that these are comparable and can be used for protein quantification in fish visceral extract.