Published Paper Details:

. Abstract: Mangroves are characteristic intertidal plant formations of sheltered tropical and subtropical coastlines and are ideal models for studying salt tolerant mechanisms at molecular level. Plant glyoxalase genes have been primarily associated with stress responses. Their over expression is known to impart tolerance to various abiotic stresses such as salinity, drought, chemicals and heavy metal toxicity. The present study made an attempt for the isolation of the genomic DNA and identification of multistress responsive Gly I gene in the mangrove plants Acanthus ilicifolius L., Avicennia officinalis L. and Bruguiera cylindrica (L.) Blume. Total cellular genomic DNA was isolated by CTAB method and its quality was checked by agarose gel electrophoresis. PCR amplification of Gly I sequence was carried out under optimal amplification profile and the quality of the gene from genomic DNA was also checked. The genomic DNA from the three mangrove plants were isolated in good quality and concentration. Under optimal PCR conditions, a prominent band of expected size was obtained in Acanthus ilicifolius (300 bp. amplicon) and Avicennia officinalis (250 bp. amplicon). The study suggests that under saline conditions more amount of glyoxalase enzyme were produced in the plant to detoxify MG and ROS which lead to the increased tolerance of these plants against salinity stress.