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Essential oils have been shown to possess antibacterial, antifungal, antiviral, insecticidal and antioxidant properties.Some oils have been used in treatment of infection in gallbladder. Essential oils are a rich source of biologically active compounds. Therefore, it is reasonable to expect a variety of plant essential oils with specific as well as general antimicrobial activity . The activity of essential oils was evaluated against five bacterial pathogens including E. coli, Salmonella sp, Klebsiella, Streptococcus, Staphylococcus (test bacteria) using disc diffusion method. Microorganisms used in this study were isolated from patients of GB diseases. Test bacteria were characterized on the basis of their biochemical characteristics. Screening of essential oils for antimicrobial activity was performed by disc diffusion method, which is normally used as rapid method to assess antimicrobial activity of essential oils . Our study have analyzed that these essential oils are useful for the preparation of herbal drugs which can be used in the treatment of infective diseases. It can also be concluded that essential oils shows good activity against test bacteria.