Published Paper Details:

Halophilic actinomycetes are promising candidate for extremozymes due to their ability to survive and function potential under extreme habitats. Among these, members of the genus Nocardiopsis are recognized for producing industrially important ?-amylases with multiple biotechnological potential. In the present study, a comparative bioinformatics analysis was carried out on eight ?-amylase producing protein sequences derived from Nocardiopsis alba and Nocardiopsis species retrieved from the NCBI database. Physicochemical characterization using ProtParam revealed that most amylases possess low theoretical isoelectric points (pI 4.13-4.71), enrichment of acidic and aliphatic residues, and low sulphur content, reflecting supporting features for adaptations to saline environments. Homology-based three-dimensional structural models were generated using SWISS-MODEL and evaluated through GMQE, QMEAN Z-scores, MolProbity, and Ramachandran plot analysis, promote the good structural reliability and stereochemical quality to remain functional active in saline habitats. These findings highlight will be used for their potential for applications in food processing, pharmaceuticals.