Published Paper Details:

Prolonged consumption of large quantities of mushrooms has led to toxicity, with toxins being the primary cause of most deaths related to mushroom poisoning. Consequently, the search for new active compounds from mushrooms has become crucial. In this study, ethanol extraction was performed on fresh fruiting bodies, and assays were conducted using commercially available onions. Observations were made from squash preparations with acetocarmine. The mitotic index, following treatment with varying concentrations of different Pleurotus extracts, showed a steady increase, except at higher concentrations of ethyl acetate. Chromosome stickiness during prophase was noted after treatment with concentrations. This stickiness was also observed at higher concentrations in Pleurotus. During metaphase, unoriented chromosomes frequently appeared after exposure to 1.5 and 2 percent of all fractions. Metaphase stickiness was induced to varying degrees at 1, 1.5, and 2 percent concentrations. Anaphase bridges were observed with higher concentrations of fractions. The study indicated that Pleurotus extracts exhibited some toxicity levels at comparable concentrations. It was concluded that prolonged and excessive use of Pleurotus might lead to chromosomal toxicity.