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ABSTRACT INTRODUCTION Breast milk is the perfect food for human infants. It is markedly different from, and uniquely superior to, artificial baby milk. Superiority of human milk is due to its superior nutritive and protective value. It provides total nutrient requirement for the first six months of life. It also prevents malnutrition and allows the child to develop fully. But when there is not enough mother's milk available due to some reasons like mother has insufficient milk glands, has had past breast surgery or is taking medication (eg: chemotherapy for cancer) and has an infection that could spread to her baby through breast feeding. For these babies an alternative must be sought. Breast milk is the main source of food for the baby, which is necessary for its growth and development. every mother looks forwards for a healthy baby. Breastfeeding also allows mother and baby to bond emotionally. WHO Recommended exclusive breastfeeding for first 6 months and continue till two years of age along with weaning. Background of the study: - Breast milk that has been expressed, pasteurized and stored in sterilized conditions is safe and healthy for babies. The milk in the human milk bank is collected from donor mothers on the third day after giving birth, if they fulfil certain safety criteria that have been set by the hospital. The milk bank has one refrigerator, one sterilizer and two pasteurizers. The steel containers are washed and sterilized in the sterilizer. The containers are then sent for collection of milk. The technician washes her hands, and labels the containers. The milk is pasteurized in the pasteurizer for half an hour at the prescribed temperature. A sample is taken and sent to microbiology department for testing for infections. After the report comes the milk is stored in the freezer at -20 degrees C. Milk thus collected can be stored for 3 to 6 months in prescribed conditions. Objectives of the study: - To assess the pretest knowledge regarding Human milk Banking among the postnatal mothers at selected rural area. To assess the effectiveness of information booklet on knowledge regarding human milk banking among the postnatal mothers at selected rural area. To determine the association between post-test knowledge scores & demographic variables among the postnatal mothers at selected rural area. Methodology: - A study was conducted on 60 among postnatal mothers a Quasi- experimental, one-group-pretest-post-test research design at selected area rural areas and the samples for the study were selected by using convenient sampling method. A tool was prepared to assess the effectiveness of informational booklet on knowledge regarding human milk banking among postnatal mothers which was divided into two parts i.e., Part 1 of demographic data and part 2 of structured questionnaire regarding knowledge on human milk bank. The content validity was done by various experts and the reliability of the tool was obtained by using Parallel Form method of reliability. A pilot study was conducted on 06 samples to check the feasibility of the study, and it was found to be feasible to conduct main study. The final data for main study was collected over 60 samples. Result: - in this study, At the time of pre-test, 35% of the postnatal mothers had poor knowledge, 61.67% of the mothers had average knowledge and 3.33% of them had good knowledge regarding human milk banking. Then post-test, no one of the postnatal mothers had poor knowledge, 28.33% of the mothers had average knowledge and 71.67% of them had good knowledge regarding human milk banking. Which, signifies the positive difference between the pretest and post-test. Conclusion: - This Study concludes that informational booklet is helpful to improve knowledge regarding human milk banking among postnatal mothers in selected rural areas.