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Published Paper Details:
Food Under The Microscope: New Frontiers In Pathogen Detection
Authors
Vinay Naik,Dr. Nitinkumar Patil,Rupali Devarkonda
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Keywords
Foodborne pathogens; PCR; ELISA; molecular diagnostics; food safety; pathogen detection.
Abstract
Foodborne pathogens remain a critical public health challenge worldwide, responsible for severe illness, hospitalizations, and economic losses. Key bacterial culprits such as Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and Bacillus cereus are frequently linked to contaminated food and water sources. While culture-based microbiological methods have long been used for pathogen identification, they are often time-consuming and lack the speed required for rapid response. This review highlights recent advancements in molecular and immunological detection methods that have revolutionized food safety monitoring. Molecular approaches--including polymerase chain reaction (PCR), real-time PCR (qPCR), multiplex PCR, loop-mediated isothermal amplification (LAMP), and reverse transcription PCR (RT-PCR)--offer rapid, sensitive, and specific identification of pathogens even at low concentrations. Immunological methods, particularly enzyme-linked immunosorbent assay (ELISA) and lateral flow assays, are valuable for their ease of use, cost-effectiveness, and potential for on-site testing. While these methods are powerful, each has limitations such as cross-reactivity, inability to differentiate viable from non-viable organisms, or dependence on specialized equipment. The integration of these approaches with biosensors, isothermal systems, and portable platforms shows promise for enhancing real-time detection capabilities. Ultimately, a combined strategy leveraging both traditional and next-generation methods is essential to prevent foodborne outbreaks and protect consumer health. This paper underscores the importance of ongoing innovation and interdisciplinary collaboration in ensuring food safety.
IJCRT's Publication Details
Unique Identification Number - IJCRT25A4722
Paper ID - 284372
Author type - Indian Author
Page Number(s) - o671-o683
Pubished in - Volume 13 | Issue 4 | April 2025
DOI (Digital Object Identifier) -
No Of Downloads - 87
Author Country - India, 421003, Ulhasnagar, Ulhasnagar, 421003, Life Sciences All
Publisher Name - IJPUBLICATION | www.ijcrt.org | ISSN : 2320-2882
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Vinay Naik,Dr. Nitinkumar Patil,Rupali Devarkonda, "Food Under The Microscope: New Frontiers In Pathogen Detection", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.13, Issue 4, pp.o671-o683, April 2025, Available at :http://www.ijcrt.org/papers/IJCRT25A4722.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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