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Background: Antibiotics have saved millions of lives and have changed the history of infectious diseases. However, in recent years, antibiotic resistance has become a major problem for global public health. Antibiotic resistance challenges the treatment of common infectious diseases, increases mortality rates and treatment costs. A study was designed to evaluate the effectiveness of structured teaching program on knowledge regarding antibiotics impact on childhood immunity among the parents at selected primary school of Surat, Gujarat with the objectives to 1) assess the knowledge of parents about antibiotics impact on childhood immunity in selected primary school, Surat City, Gujarat. 2) assess effect of structured teaching programmed regarding antibiotics Impact on childhood immunity among parents of Surat City, Gujarat.3) To find out the association of the knowledge of primary school parents regarding antibiotic impact on childhood immunity with their selected demographic variables. Methods: A Quantitative Research approach and pre-experimental one group pre-test post- test research design with non-probability convenient sampling technique was used to collect data from 60 parents of Children (Age group between 5 to 8 years) in selected primary school of Surat, Gujarat. A demographic tool, structured knowledge questionnaire was used to describe the findings. Result: Analysis and interpretation of data collected from 60 samples, on antibiotic impact on childhood immunity and its interpretation among parents of selected primary schools of Surat. Knowledge was assessed by using descriptive and inferential statistics. The data gathered were summarized in the master sheet. Findings revealed that the level of knowledge regarding antibiotic impact on childhood immunity through pre-test and post-test. In pre-test out of 60 parents 34(56.66%) of parents had poor knowledge, 11(18.33%) of parents had average knowledge, 16(26.66%) of parents had good knowledge with regard to post score 5(8.33%) parents had poor knowledge, 15(25%) of parents had average knowledge, 40(66.66%) of them had good knowledge. The paired t-test results t=10.457. Since P-value=2.0015> 0.05 reveals the effectiveness of the Awareness package. The calculated value is higher than the tabulated value. The study aims to assess the knowledge and evaluate the effectiveness of the structure teaching programme on knowledge regarding antibiotics and impact on childhood immunity among parents of primary school children's around 60 samples were selected using Non probability purposing sampling technique. Data was generated and analysed by descriptive and inferential statistics. The paired 't' test value of the study was 19.5 at the significance of 0.05. The calculated value is higher than tabulated (p-value) = 2.00. This shows highly effectiveness of the structured teaching programme among selected samples. Association between pre-test knowledge score with their socio demographic variables regarding antibiotic impact on childhood immunity among parents of primary school children in selected school of Surat. Hence, it was assessed that Age, gender, No. Of children, Education of family, information, occupation, leaving the area does not have any significant association (Age ?2= 7.14, gender ?2 = 5.74, Number of children ?2 = 10.87, Education of head of family ?2= 4.22, Source of Information regarding antibiotics ?2 = 4.26, occupation of family head ?2=10.79, living area ?2= 4.50) and Monthly income ?2= 6.36. Other demographic variables such as Type of Family do have significant association in the study (Type of family ?2= 16.25) Conclusion: The study was conducted to assess the effectiveness of structure teaching program on knowledge regarding antibiotics impact on childhood immunity among the parents at selected primary school of Surat, Gujarat. The finding of the study showed that structured teaching program about antibiotic impact on childhood immunity was effective among parents of primary school children. Association between pre-test knowledge score and socio demographic variables regarding antibiotic impact on childhood immunity among parents of primary school children in selected school of Surat. Hence, it was assessed that, Type of Family and Monthly Income does have significant association in the study.