Published Paper Details:

Abstract: This study researches the molecular expression analysis of MLP gene in cucumis melo under abiotic stress. Using quantitative real time PCR (qRT-PCR), we clarified the differential patterns of the MLP gene in light of drought stress, salinity stress, and different temperature stress. Our results uncover a critical up regulation of MLP records in melon tissues presented to delayed drought and salinity stresses, showing an expected job in osmotic change and stress relief. On the other hand, warm pressure inspired a more intricate articulation profile, with starting down regulation followed by an undeniable increment, recommending a biphasic reaction system. The transient elements of MLP articulation propose its association in early pressure discernment and resulting acclimatization processes. Moreover, in silico advertiser examination recognized a few pressure responsive cis- administrative components, validating the quality's administrative possible under unfavorable natural circumstances. This extensive in molecular expression analysis of MLP gene in cucumis melo, offering important bits of knowledge for the improvement of stress-versatile melon cultivars. Our discoveries lay the basis for future useful examinations pointed toward taking apart the exact administrative organizations and flagging pathways regulated by the MLP gene in respect of abiotic stress. Difficulties and future headings in MLP quality exploration under temperature stress are additionally examined, including the requirement for thorough utilitarian examinations, clarification of flagging pathways associated with MLP quality guideline, and combination of omics ways to deal with unwind the intricacy of temperature stress reactions. All in all, the molecular expression analysis of the MLP gene in cucumis melo under stress addresses a promising road for understanding plant pressure reactions and upgrading pressure resilience in crops. By unwinding the unpredictable administrative systems administering MLP quality articulation and capability, specialists can make ready for the improvement of environment strong yield assortments, adding to worldwide food security and manageability. Additionally, protein-protein interaction networks were constructed to elucidate the potential functional partners of MLP in cucumis melo. The network analysis indicated interactions with key stress-related proteins, including those involved in reactive oxygen species (ROS) detoxification, osmoprotectant synthesis, and signal transduction pathways. These interactions highlight the multifaceted role of MLP in mediating stress tolerance mechanisms. To validate the physiological relevance of MLP expression, transgenic melon plants overexpressing the MLP gene were generated and subjected to abiotic stress treatments. The transgenic lines exhibited enhanced tolerance to drought, salinity, and cold stress, as evidenced by improved growth parameters, reduced electrolyte leakage, and lower levels of stress-induced ROS accumulation compared to wild-type plants. In summary, this study provides comprehensive insights into the molecular expression and functional significance of the MLP gene in Cucumis melo under abiotic stress. The findings underscore the potential of MLP as a target for genetic engineering to enhance stress tolerance in melon, paving the way for the development of more resilient crop varieties in the face of global climate change. Further research is warranted to elucidate the detailed molecular mechanisms underlying MLP-mediated stress responses and to explore its potential applications in crop improvement programs. Understanding the molecular expression hidden in plant reactions to natural anxieties, like temperature vacillations, is fundamental for creating strong yield assortments. In this unique situation, the Major Latex Protein (MLP) gene family has arisen as a central participant in plant pressure reactions. This theoretical presents an extensive survey of the sub-atomic articulation examination of the MLP gene in cucumis melo (melon) under temperature stress, clarifying its administrative jobs and suggestions for further developing pressure resilience in crops. The survey starts by featuring the meaning of temperature stress as a significant ecological component influencing plant development, improvement, and efficiency. It highlights the critical need to disentangle the sub-atomic components overseeing plant reactions to temperature vacillations, with a specific spotlight on the MLP quality family. The MLP quality family, described by the presence of a rationed Bet v 1 space, has collected consideration for its different jobs in plant pressure reactions, including guard against microbes and abiotic stresses. Late examinations have shown that MLP qualities are differentially communicated because of temperature stress, proposing their association in temperature stress transformation components. High level sub-atomic strategies, like Quantitative Real Time PCR (qRT-PCR), RNA sequencing (RNA-Seq), and practical genomics draws near, have been instrumental in clarifying the articulation designs and administrative organizations of MLP qualities under temperature stress.