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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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Phytochemical Analysis and Thin Layer Chromatography (TLC) of Moringa Oleifera Methanol, Ethanol, Water, and Ethyl Acetate Extracts
Authors
Ghavate Siddhi Ashok,Bhosale Gitanjali Pandurang,Bochare Shrinath Ranganath,Sakore Asmita
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Keywords
TLC,Moringa Oleifera,Phytochemical analysis,RF value
Abstract
The phytochemical composition of several medicinal plants has been examined. One of these plants is Moringa oleifera. It is widespread in tropical and subtropical regions. It has vital medical applications as well as a high nutritional value. The primary goal of this work was to conduct a preliminary phytochemical screening to determine the key classes of bioactive chemicals found in Moringa oleifera dry leaves. Many solvents (Methanol, ethanol, ethanol acetate, and water) utilized to select the optimal solvent can be employed for extraction and TLC profiling of all sequential extracts. TLC was performed in silica gel plates using various mobile phase methods, including chloroform: methanol: ethanol (1:1:1), (2:2:0.5), and chloroform: glacial acetic acid: methanol (4:5:1) chloroform:methanol:ethyl acetate(1:1:1), trichloro acetic acid:ethyl acetate:methanol(1:7.5:1.5). All solvent extracts contained alkaloids, flavonoids, tannins, and phenols, according to phytochemical analysis of four extracts. The concentration of these chemicals, however, is higher in ethanol and ethanol acetate extracts (+++).Thin layer chromatographic investigations of Moringa olifera leaf extract (methanol, ethanol, ethanol acetate, and water) revealed many colored phytochemical substances with varying Rf values. Methanol and ethyl acetate extracts performed best, yielding four bands with Rf less than one, whereas ethanol extract yielded five bands with Rf less than one and water extract yielded none.
Furthermore, with various solvent extracts, the other mobile phases detect less bands. The current study found that employing ethanol and ethyl acetate as solvents increased the content of bioactive components in Moringa oleifera leaf extracts. The solvent is more expensive than the other solvents. TLC, on the other hand, revealed four bands with higher Rf when methanol was used. Extract of ethyl acetate. In conclusion, methanol and ethyl acetate extracts of Moringa oleifer leaves possess a greater concentration ofBioactive component content, which can be employed in future research on this plant.
IJCRT's Publication Details
Unique Identification Number - IJCRT2312242
Paper ID - 247630
Author type - Indian Author
Page Number(s) - c76-c82
Pubished in - Volume 11 | Issue 12 | December 2023
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No Of Downloads - 89
Author Country - India, 412307, Pune , Pune , 412307, Pharmacy All
Publisher Name - IJPUBLICATION | www.ijcrt.org | ISSN : 2320-2882
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Ghavate Siddhi Ashok,Bhosale Gitanjali Pandurang,Bochare Shrinath Ranganath,Sakore Asmita, "Phytochemical Analysis and Thin Layer Chromatography (TLC) of Moringa Oleifera Methanol, Ethanol, Water, and Ethyl Acetate Extracts", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.11, Issue 12, pp.c76-c82, December 2023, Available at :http://www.ijcrt.org/papers/IJCRT2312242.pdf
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