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Real-time Polymerase Chain Reaction (PCR) in the microbiology laboratory
Authors
Dr.Prashant Gupta,Mrs.Akanksha Awasthi,Mr.Sunny Patel,Ms.Sujata Katiyar,Ms.Shreya Bhardwaj
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Keywords
Microbiological,Diagnostic,Forensic,RealtimePCR,contamination,poc,biomedicine,labor intensive, Transcription, Commercialization
Abstract
Use of PCR techniques in the field of Microbiological, diagnostics and Forensic Science has increased to the point where it is now accepted as the standard method for detecting nucleic acids from a number of sample and microbial types.In the scientific laboratory, traditional PCR was already a necessary instrument, though. Due to its increased speed, sensitivity, and reproducibility as well as its reduced danger of carryover contamination, real-time PCR has facilitated the wider use of PCR. During real-time PCR, a growing variety of chemistries are employed to detect PCR products as they build within a closed reaction vessel. PCR is being used in two key areas in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also address the commercialization of these techniques before concluding with an examination of their modifications and application in novel areas of biomedicine.. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. The gold standard for SARS-CoV-2 diagnosis is real-time reverse transcription PCR. The dynamic range of initial target molecule determination in real-time PCR is very large (at least five orders of magnitude). Real-time quantitative PCR is more accurate and requires less labor than traditional quantitative PCR procedures.
IJCRT's Publication Details
Unique Identification Number - IJCRT2312160
Paper ID - 247445
Author type - Indian Author
Page Number(s) - b389-b397
Pubished in - Volume 11 | Issue 12 | December 2023
DOI (Digital Object Identifier) -
No Of Downloads - 53
Author Country - India, 243001, BAREILLY, BAREILLY, 243001, Science and Technology
Publisher Name - IJPUBLICATION | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
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Dr.Prashant Gupta,Mrs.Akanksha Awasthi,Mr.Sunny Patel,Ms.Sujata Katiyar,Ms.Shreya Bhardwaj, "Real-time Polymerase Chain Reaction (PCR) in the microbiology laboratory", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.11, Issue 12, pp.b389-b397, December 2023, Available at :http://www.ijcrt.org/papers/IJCRT2312160.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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