Published Paper Details:

Aim of the present piece of work was to biochemically segregate the different amino acids like essential amino acids (EAA), non-essential amino acids (NEAA) in proteins and fatty acids like saturated fatty acids (SFA); mono-unsaturated fatty acids (MUFA) and poly saturated fatty acids (PUFA) of fats in the muscle tissues of three commercially less importance fresh water fishes like Clarias batrachus, Channa punctatus and Anabas testudineus. The parameters under study were estimated in all the three species irrespective of sex, size and maturity. Nine essential amino acids namely Lysine, Leucine, Valine, Isoleucine, Threonine, Phenylalanine, Methionine, Tryptophan and Histidine that are very important for human health were found to be present in all the species examined. Among all the essential amino acids, Leucine is found to be highest in content i.e. 1.29mg, 0.86mg and 1.38mg/100 gm fish muscle among the above three species respectively. However, in NEAA; Glutamic acid content was recorded to be highest in C.batrachus and C.punctatus with value of 2.41mg and 0.79 mg/100 gm tissue; whereas, Cysteine value of 2.35 mg was recorded in A.testudineus. Glutamic acid plays an important role in amino acid metabolism because of its role in transamination reactions and is necessary for the synthesis of key molecules, such as glutathione which are required for removal of highly toxic peroxides and the polyglutamate foliate cofactors. The highest value in SFA of C21:0 was found to be 0.45mg in C. batrachus, whereas the C16:0 content was recorded to be 1.07mg and 3.59mg in C.punctatus and A.testudineus respectively. Similarly, the highest value of MUFA in C18:1n9 has found in C.batrachus and C.punctatus with a value of 1.54mg and 1.01mg per 100gm tissue respectively. The MUFA of C18:1n9t has highest value found in A.testudineus of 1.29 mg. The PUFA like Omega 6 (? 6) content was recorded highest among above three species with a value of 0.89mg, 0.98mg and 0.69 mg per 100gm of tissues. The total EAA were found to be 7.07mg, 5.19mg, and 7.42mg; whereas the value of NEAA was recorded to be 7.16mg, 4.29mg, and 9.58mg per 100 gm tissue differ between the species. However the total SFA was recorded 1.909mg, 3.25mg, and 4.283mg ; MUFA 2.135mg, 3.650mg, and 1.876mg and the total PUFA content was found to be 2.990mg, 3.402mg, and 1.975mg respectively in the fishes under study. Docosahexaenoic (DHA) and Eicosapentaenoic acid (EPA) were the dominant polyunsaturated fatty acid predominantly found in all the species. The results indicate that all the fish species under experimentation contain high quality of protein and fats with necessary fatty acids and many essential amino acids for normal development and growth of human and can be exploited as alternative fish source like that of commercial important variety. Among all the species under study, A.testudineus was found to be the best choice among all the species under study as it contains high nutrient components and the perfect ratio of SFA, MUFA and PUFA followed by C.batrachus and C.punctatus.