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GENETIC CHARACTERIZATION OF COLOURED RICE GENOTYPES (ORYZA SATIVA L.) FROM DIFFERENT REGIONS OF INDIA USING MOLECULAR AND BIOCHEMICAL MARKERS
Authors
Dr. Amruta Desai,Dr. Bhushan Aher,Dr. Subhash N.
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Keywords
Red rice, SSR, INDEL, Polymorphism, Genetic diversity
Abstract
Genetic diversity analysis of 24 coloured and 8 white rice genotypes were conducted usingSSR and InDel markers. Among them, SSR markers such as RM5, RM 210, RM 251, RM 261, RM 490, RM 3859 and InDel marker RID 12, RID 13, RID 19 and RID 25 were capable to distinguish coloured rice genotypes. The PIC values ranged from 0.16 (RM 232) to 0.78 (RM 258) and 0.52 (RID 25) and 0.80 (RID 12) for SSR and INDEL markers respectively. The DICE similarity coefficient based on SSR and InDel analysis was 0.89 and 1.00, respectively while the cophenetic correlation (r) value for cluster analysis of SSR and InDel was 0.89 and 0.71, respectively. Molecular variance (AMOVA), F statistics and genetic variation statistics revealed that coloured rice genotypes were more genetically diverse than white rice genotypes. In the case of both SSR and INDEL markers highest genetic diversity was observed between Pankhali 203 (White pericarp) and IRST 1 (Red pericarp) whereas least genetic diversity was observed between RRT GR12 (Red pericarp) and Krishna Kamod (White pericarp). However, it was also found that black and brownpericarp isgeneticallysimilar to red pericarp. It was suggested from the conducted experiment to adopt SSR markers as it gave better results than InDel even though all InDel markers used in present study were also able to distinguish coloured rice genotypes.
IJCRT's Publication Details
Unique Identification Number - IJCRT1704430
Paper ID - 170494
Author type - Indian Author
Page Number(s) - 3234-3245
Pubished in - Volume 5 | Issue 4 | December 2017
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No Of Downloads - 1224
Author Country - 391 155, India, Jabugam, Jabugam, India, Biological Science
Publisher Name - IJPUBLICATION | www.ijcrt.org | ISSN : 2320-2882
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Dr. Amruta Desai,Dr. Bhushan Aher,Dr. Subhash N., "GENETIC CHARACTERIZATION OF COLOURED RICE GENOTYPES (ORYZA SATIVA L.) FROM DIFFERENT REGIONS OF INDIA USING MOLECULAR AND BIOCHEMICAL MARKERS", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.5, Issue 4, pp.3234-3245, December 2017, Available at :http://www.ijcrt.org/papers/IJCRT1704430.pdf
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