Published Paper Details:

Brachymenium bryoides is an important genus of moss generally regarded as an indicator plant to understand the effects of climate change. In Darjeeling Himalayan region, like any other biodiversity hotspots, the moss flora is very rich, although neglected. Any biochemical work on these plants, especially the present species is absolutely lacking. The presence of enzyme L-myo-inositol-1-phosphate synthase (MIPS) is essential for the survival of this moss like any other groups of plants since it forms the cell walls as well as the sexual units in plants. The enzyme MIPS from Brachymenium bryoides utilizes D-glucose-6-phosphate as a substrate NAD+ as a co-factor respectively. It showed a pH optimum at 7.5 while the temperature maximum was at 40S?C. The enzyme activity was remarkably stimulated by Ca2+, NH4+ and Mg2+ and extremely inhibited by Zn2+, Cd2+, Mn2+ and Hg2+. The Krn values for D-glucose-6-phosphate and NAD+ were found to be as 6.72 mM and 0.78 mM respectively while the Vrnax values were 1.62 mM and 1.11 mM for D-glucose-6-phosphate and NAD+ respectively.