Published Paper Details:

SP3 Transcription factor contains 81,925 Dalton mass, which member of the Kruppel like zinc finger protein family that is clinically relevant for many neuronal transmission diseases. Considering the functional importance and lack of X-ray crystal structure of SP3 TFs protein, present work was undertaken to build the 3D structure of a protein using homology modeling with a multi-template approach. This present study, we chose three different SP3 templates (PDB ID: 3EBT, 4M9E, and 2WBS) were used for homology modeling. Five models were developed with the help of multiple sequence alignment respect to templates using Modeller 8.0.0 software. All models were refined and ranked as per their overall DOPE-score. The top-ranked predicted model of SP3 TFs had 93.8% of residues in favored regions as revealed by Ramachandran plot and the ERRAT score was 100% which indicated an accurate model. The results of the homology modeling study and the proposed model can be further used for understanding the structural and functional characteristics of SP3 and to gain more insights to the molecular basis of SP3 inhibition through docking and molecular dynamics simulation studies.